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Abstract
The article presents results from determining soluble protein content in plant samples of Prunus spinosa L. using the Bradford method and assessing their comparative characteristics. The study used samples obtained from the leaves of P. spinosa. Protein extraction was performed using a 50 mM phosphate buffer solution at pH 7.0. To reduce the potential influence of phenolic compounds in plant tissues, we used polyvinylpolypyrrolidone (PVPP) during extraction. The obtained extracts were centrifuged, and the soluble protein content in the clear supernatant was determined using the Bradford colourimetric method. We used a series of bovine serum albumin (BSA) standard solutions to calculate protein concentration and construct a calibration curve.
The study showed that soluble protein content in P. spinosa samples varies by plant organ and by individual sample characteristics. We compared the optical density values of leaf and fruit extracts and calculated protein concentrations from these measurements. The Bradford method enabled a comparative assessment of soluble protein content in plant extracts. The calibration curve constructed using BSA standards served as the basis for quantitatively determining protein concentrations in the studied samples.
The results provide an opportunity to assess the distribution patterns of soluble proteins in P. spinosa tissues and to compare leaf samples. The findings may serve as baseline data for characterising the biochemical properties of P. spinosa and for planning further biochemical studies of the protein composition of plant materials.